human bj fibroblast cell line  (ATCC)

Journal: NPJ Science of Food

Article Title: From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients

doi: 10.1038/s41538-026-00723-9

Figure Lengend Snippet: Evaluation of the effects of the RVs and CVs on H 2 O 2 -induced reactive oxygen species (ROS) and MDA production levels in fibroblast BJ-5TA cells ( A , B ) and differentiated C2C12 ( C , D ) cells. The data points represent the averages ±SD of three independent experiments in duplicate. All data sets were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Different lowercase letters indicate a significant difference ( p < 0.05) between different treatments. Control: untreated cells. RVs: rosemary vesicles; CVs: coffee vesicles. 7.8 ×10 6 vesicles/mL and 39 ×10 6 vesicles/mL correspond to 0.1 and 0.5 mg nanovesicles/mL.

Article Snippet: hTERT-immortalized human skin fibroblasts BJ-5TA cell line was bought from ATCC (ATCC (CRL-4001 TM), from LGC Standards, Milan, Italy), cultured in DMEM high glucose and Medium 199 (4:1 ratio) with stable L-glutamine supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin and incubated at 37 °C under a 5% CO 2 atmosphere.

Techniques: Control

Journal: Microorganisms

Article Title: Saponin Improves Recovery of Bacteria from Orthopaedic Implants for Enhanced Diagnosis Ex Vivo

doi: 10.3390/microorganisms13040836

Figure Lengend Snippet: Novel 3D PJI Soft Tissue Model. ( A ) CLSM images demonstrating the presence of S. epidermidis biofilm aggregates (turquoise) on the TAV discs, surrounded by human cells and host tissue (red, collagen) and single S. epidermidis colonies (green) within the host tissue matrix colonized by human fibroblasts (red). Scale bars indicate 50 µm. ( B ) Fluorescent images obtained from the tissue model with the Moleculight i: X TM device showing the host tissue (green, collagen) surrounding the 1 cm TAV discs. Red signal indicates S. epidermidis on the implant and in the tissue (arrows). ( C ) S. epidermidis CFU recovery of infected tissue models. The implant disc and tissue were collected and treated separately either with 0.001% ( w / v ) saponin in PBS combined with vortexing or saline sonication, n = 5 replicates. Abbreviations: CFUs, colony-forming units; CLSM, confocal laser scanning microscopy; TAV, titanium alloy Ti-6AI-4V.

Article Snippet: To do so, the human skin fibroblast cell line BJ (ATCC, Manassas, VA, USA) was grown in DMEM/F12 + 5% FCS until confluent and then harvested.

Techniques: Infection, Saline, Sonication, Confocal Laser Scanning Microscopy